Insulin ELISA
Insulin ELISA
(10-1113-01)
Kit format: 1 x 96 wells
Measurement range: 3 - 200 mU/L (0.13 - 8.70 μg/L)
1.Calibrated against 1st International Reference Preparation 66/304 for human insulin
2.No cross-reactivity to C-peptide or Proinsulin des(31-32) or split (32-33)
3.Insignificant or no cross-reactivity to insulin analogs
4.CE/IVD labeled on select markets
5.Controls available: 10-1134-01 (US only) or 10-1164-01
DESCRIPTION
A high quality ELISA for specific measurement of human insulin in serum, plasma or cell culture media.
The Mercodia insulin assays have little or no cross-reactivity to insulin analogs or to rat or mouse insulin and comes with full cross-reactivity data.
The Mercodia Insulin ELISAs are the only ELISA assays to satisfy the total error requirements in the Insulin Standardization Program.
TEST CHARACTERISTICS
SPECIES：Human
SAMPLES：Serum, plasma, cell culture medium
SAMPLE VOLUME：25μL
ASSAY RANGE：3 - 200 mU/L (0.13 - 8.70 μg/L)
DETECTION LIMIT：≤ 1 mU/L (0.043 μg/L)
INCUBATION：1h + 15 min
DETECTION METHOD：Absorbance
SPECIFICITY
The following cross-reactions have been tested:
C-PEPTIDE：<0.01 %
PROINSULIN：<0.01 %
PROINSULIN DES (31-32)：<0.5 %
PROINSULIN SPLIT (32-33)：<0.5 %
PROINSULIN DES (64-65)：98 %
PROINSULIN SPLIT (65-66)：56 %
INSULIN ASPART：4 %
INSULIN DETEMIR：Not detected
INSULIN GLARGIN：24%
INSULIN GLULISINE：Not detected
INSULIN LISPRO：Not detected
IGF-I：<0.02 %
IGF-II：<0.02 %
RAT INSULIN：0.7%
MOUSE INSULIN：0.3 %
PORCINE INSULIN：93 %
OVINE INSULIN：48 %
BOVINE INSULIN：31 %
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